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  (Photo: Reuters/Brendan McDermid)
  Pop sensation Justin Bieber

Justin Bieber has been accused of cursing out one of his beloved fans while he was at Disney Land on Valentine's Day, and the incident is rumored to have cost him thousands of Twitter followers.

The 17-year-old pop icon, renowned for his innocence and strong Christian values, can be heard screaming "**** You!" in response to a giggly female fan who had said "I follow you on Twitter!" according to HollywoodLife.

In a 23 second video clip, which went viral on social networking site YouTube, a blurry Bieber is seen on a ride and heard screaming the profanity which left the girl stunned and she is later heard saying, "I won't follow you anymore ***hole."

Bieber is believed to have been at the amusement park with long-term girlfriend, Selena Gomez, along with family members and it is still unclear why the "Believe" singer lost his cool.

Although fans expected the happy couple to spend Valentine's Day only with each other, they were spotted strolling through Disneyland with Bieber's mother Pattie Mallette, and his younger brother and sister, Jaxon and Jazmyn.

Fans took to twitter to weigh in on Bieber's uncharacteristic verbal attack on the unidentified fan, and one of the singer's fan pages claims that his actions have led some fans to retaliate.

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"Justin lost seriously 10k followers, when he said "**** you" to a pathetic hater who called him an ***hole. lol k more bieber for me," the "Ask Justin" fan page tweeted.

"Justin Bieber said **** YOU [to] fans when they asked for a photo?! WHAT IS HAPPENING WITH YOU?!" Alesya Villegas tweeted.

"What the **** is this? Justin's getting a little too cocky I think," Deanne tweeted.

The news comes shortly after Bieber granted 6-year-old Avalanna Routh's wish to "marry" him for Valentine's Day, after learning that she suffers from an aggressive form of brain cancer called ATRT (Atypical Teratoid Rhabdoid Tumor).

While the two were not legally married, the self proclaimed "mrs bieber" had said that just meeting and spending time with Bieber would signify that they were married and the pop star happily obliged.

"That was one of the best things i have ever done. she was AWESOME! Feeling really inspired now!" Bieber tweeted, later saying "#MrsBieber really inspired me."

Bieber has not yet responded to the accusations.

SEE VIDEO OF BIEBER INTERACTING WITH FANS TO MAKE A DIFFERENCE